Recognition of point mutations in codon 12 of the K-ras gene is useful for the early diagnosis of several types of cancer in humans, including colorectal cancer. A microfluidic analyzer, that can detect low-abundance point mutations in human genomic DNA, was developed using a modular approach. Each functional device, including a microfluidic polymerase chain reaction (PCR) reactor, a passive diffusional micromixer reactor, and a microfluidic ligase detection reaction (LDR) reactor, was designed and fabricated in ...
1 Jan 2009
Chemical and Biological Microsystems Society